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American Society of Microbiology ABMM Sample Questions:

1. A patient develops a severe, rapidly progressive pneumonia with hemoptysis. Gram stain of the sputum shows numerous small, pleomorphic Gram-negative coccobacilli. Growth on Bordet-Gengou agar is observed. The MOST likely causative agent is:

A) Moraxella catarrhalis
B) Bordetella pertussis
C) Legionella pneumophila
D) Haemophilus influenzae

2. A patient with a history of intravenous drug use develops a bloodstream infection with Staphylococcus aureus. Antimicrobial susceptibility testing reveals resistance to methicillin. The mechanism of methicillin resistance in Staphylococcus aureus is primarily mediated by:

A) Production of beta-lactamase enzymes.
B) Alteration of penicillin-binding proteins (PBPs).
C) Acquisition of genes encoding aminoglycoside-modifying enzymes.
D) Increased expression of efflux pumps.

3. A foodborne outbreak is suspected after several individuals who attended a potluck dinner develop nausea, vomiting, and abdominal cramps within 1-6 hours of eating. No fever or diarrhea is reported. The MOST likely bacterial toxin involved is:

A) Botulinum toxin
B) Staphylococcal enterotoxin
C) Shiga toxin
D) Clostridium perfringens enterotoxin

4. A traveler returning from sub-Saharan Africa presents with cyclical fevers, chills, and sweats. A Giemsa- stained peripheral blood smear reveals multiple ring forms within red blood cells, some applique forms, and occasional banana-shaped gametocytes. No Schüffner's dots or red blood cell enlargement is observed. Which Plasmodium species is the most likely cause?

A) Plasmodium ovale
B) Plasmodium malariae
C) Plasmodium vivax
D) Plasmodium falciparum

5. A clinical microbiology laboratory is investigating a cluster of bloodstream infections caused by Staphylococcus aureus. To differentiate between community-associated (CA-MRSA) and healthcare- associated (HA-MRSA) strains, they may look for the presence of:

A) The mecA gene, which is common to both CA-MRSA and HA-MRSA.
B) Specific staphylococcal cassette chromosome mec (SCCmec) types, such as SCCmec IV or V inCA- MRSA.
C) Panton-Valentine leukocidin (PVL) toxin genes, which are exclusively found in HA-MRSA.
D) Resistance to non-beta-lactam antibiotics, which is more common in CA-MRSA.

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